公开(公告)号：US20250163499A1

公开(公告)日：2025-05-22

申请号：US18938479

申请日：2024-11-06

Applicant: Soochow University ,  Suzhou Shuda Innovation Medical Technology Co., Ltd.

Inventor： Min Fei ,  Yingchao Wu ,  Weiwei Li ,  Yifeng Cao ,  Kaiye Lu ,  Waner Yan ,  Zhaoqin Fei ,  Dongning Zhu ,  Lijun Lu

IPC: C12Q1/6827 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6876

Abstract: Provided are a primer and probe composition for cat blood type detection, a detection method, and use. The primer and probe composition for detecting the wild-types and the mutants of the above four sites is designed according to the single nucleotide polymorphism (SNP) mutation of genotyping of 179G>T, 268T>A, 364C>T and 1322delT four sites of a cat cytidine monophospho-N-acetylneuraminic acid (CMAH) gene; an amplification curve is plotted by utilizing a real-time fluorescence polymerase chain reaction (PCR) technology, and the recognition of bases of four SNP sites is achieved by analysis of the amplification curve, so as to determine cat blood types. The detection method of the present disclosure is carried out entirely in a closed space, thereby avoiding the problem of environmental aerosol pollution.

公开(公告)号：US20250154600A1

公开(公告)日：2025-05-15

申请号：US18839936

申请日：2023-03-06

Applicant: CEDARS-SINAI MEDICAL CENTER

Inventor： Hisashi TANAKA ,  Michael M. MURATA ,  Armando E. GIULIANO

IPC: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: Disclosed herein are a method of detecting invasive tumor in a subject and a method of treating a subject with cancer based on invasiveness of tumor. In various embodiments, the method of detecting tumor invasiveness includes denaturing genomic DNA isolated from a tumor sample obtained from the subject; renaturing the denatured DNA for tumor DNA palindrome to form a snap back DNA; digesting the renatured DNA with a nuclease that digests single strand DNA; amplifying the tumor DNA palindrome by adapter ligation-mediated polymerase chain reaction (PCR) with genome-wide analysis of Palindrome Formation (GAPF); performing a sequence scan across multiple samples of the amplified tumor DNA palindrome; mapping reads of GAPF-seq from the sequence scan into a plurality of bins; quantifying reads in each bin; and determining whether the tumor sample is an invasive tumor based on GAPF profiles generated by analyzing the quantified reads in each bin.

公开(公告)号：US20250154589A1

公开(公告)日：2025-05-15

申请号：US19030426

申请日：2025-01-17

Applicant: Twist Bioscience Corporation

Inventor： Bill James Peck

IPC: C12Q1/6874 ,  B01J19/00 ,  C12Q1/6806

Abstract: Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Additionally, devices described herein for de novo synthesis of oligonucleic acids encoding information related to the original source information may have a flexible material for oligonucleic acids extension.

公开(公告)号：US20250154572A1

公开(公告)日：2025-05-15

申请号：US18838533

申请日：2023-02-17

Applicant: University of Virginia Patent Foundation

Inventor： James P. Landers ,  Leah Michele Dignan ,  Michael Shane Woolf

IPC: C12Q1/6844 ,  B01L3/00 ,  C12Q1/6806

Abstract: Apparatus and techniques described herein can include or use a rotationally-driven microfluidic assembly. For example, a sample can be propelled to a sample recovery chamber from a sample chamber using a gas evolved from a reaction between the liquid reagent and a dry reagent. Such gas evolution can provide displacement of a sample liquid or other liquid in an inward direction, such as proximally toward a center of rotation. Such gas evolution can include features or reagents, or both, that are compatible with downstream nucleic acid amplification tests.

公开(公告)号：US12297490B2

公开(公告)日：2025-05-13

申请号：US17271815

申请日：2019-08-28

Applicant: SOPHIA GENETICS S.A.

Inventor： Alisa Alekseenko ,  Vicente Jose Pelechano Garcia

IPC: C12Q1/6855 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods and products are disclosed for asymmetrically adapting fragmented nucleic acids for next generation sequencing, including providing strand identifier sequences and index sequences to identify the source strand and sample, respectively, of the fragmented nucleic acids. The methods and products allow for efficient and reliable detection of low-frequency mutations including in subpopulations of cells within a subject and also for the amplification of the fragmented nucleic acids when there is a low yield of isolated fragmented nucleic acids.

公开(公告)号：US12297484B2

公开(公告)日：2025-05-13

申请号：US18341446

申请日：2023-06-26

Applicant: Genefirst Ltd.

Inventor： Guoliang Fu ,  Thomas Dunwell

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: This invention relates to methods, compositions and kits for extending a polynucleotide and for preparing sequencing library of polynucleotides involving generating modified target polynucleotide on an adaptor template oligonucleotide and tagging one or two strands of a target sequence. The sequencing library is suitable for massive parallel sequencing and comprises a plurality of double-stranded nucleic acid molecules.

公开(公告)号：US20250140343A1

公开(公告)日：2025-05-01

申请号：US18930793

申请日：2024-10-29

Applicant: Myriad Women's Health, Inc.

Inventor： Ashley Acevedo ,  Christopher J. Battey

IPC: G16B20/20 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B35/20

Abstract: Described herein are methods of preparing an enriched library of nucleic acids, comprising: (a) identifying a panel of patient-specific somatic variants present in a tumor sample from a patient, wherein the somatic variants comprise one or more of (i) tumor somatic variants, (ii) non-tumor somatic variants, and (iii) germline sites incorrectly identified as somatic; (b) preparing a sample of cell-free DNA fragments from the patient for sequencing; (c) selectively enriching the cell-free DNA for the fragments comprising one or more of the somatic variants to generate an enriched library; and (d) analyzing the enriched library by generating sequencing reads for each somatic variant position, wherein analyzing comprises applying a classification model to the somatic variants to classify each somatic variant as either tumor, non-tumor, or germline variants.

公开(公告)号：US20250137047A1

公开(公告)日：2025-05-01

申请号：US18837700

申请日：2023-02-13

Applicant: Miltenyi Biotec B.V. & Co. KG

Inventor： Robert Pinard ,  Michel Perbost ,  Matthias Bernhard Wahl

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6841 ,  C12Q1/6876

Abstract: The invention is directed to a method to synthesize oligonucleotides on the surface of a biological sample comprising the steps a. Binding a plurality of primer molecules to spatial locations on the surface of the biological sample with a stochastic surface distribution thereby creating a oligonucleotides bound to the biological sample b. providing the biological sample with A, T, C or G nucleotides having a protecting unit at their 3′ positions c. incorporating one of the A, T, C or G nucleotides having a protecting unit at their 3′ positions at the 3′end of at least one oligonucleotides bound to the biological sample by addition of a terminal transferase thereby extending the oligonucleotides d. adding at least one photo-activated cleave agent capable of removing the protection unit from the incorporated protected nucleotide e. removing the protecting unit from the incorporated protected nucleotide by activating the photo-activated cleave agent with light provided to at least one spatial location of the biological sample f. Repeating steps b) to e) to incorporate further nucleotides to at least one oligonucleotide.

公开(公告)号：US20250137025A1

公开(公告)日：2025-05-01

申请号：US18940636

申请日：2024-11-07

Applicant: 10x Genomics, Inc.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Zahra Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20250136971A1

公开(公告)日：2025-05-01

申请号：US18657366

申请日：2024-05-07

Applicant: The Regents of the University of California

Inventor： Hugh E. Olsen ,  Miten Jain ,  Mark A. Akeson

IPC: C12N15/10 ,  C12N15/11 ,  C12Q1/6806 ,  C40B30/04 ,  C40B40/06 ,  C40B70/00

Abstract: Provided are methods of producing size-selected nucleic acid libraries. The methods include contacting a nucleic acid sample and a nucleic acid binding reagent including an affinity tag, under conditions in which nucleic acids of less than a desired length are substantially bound to the nucleic acid binding reagent and nucleic acids of the desired length are substantially not bound to the nucleic acid binding reagent. The conditions include the duration of the contacting, the concentration of the nucleic acid binding reagent, or both. The methods further include separating, using the affinity tag, the nucleic acids of less than the desired length bound to the nucleic acid binding reagent from the nucleic acids of the desired length not bound to the nucleic acid binding reagent, to produce a size-selected nucleic acid library. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure, are also provided.