公开(公告)号：US09388427B2

公开(公告)日：2016-07-12

申请号：US12320434

申请日：2009-01-26

Applicant: Lakshman R. Sehgal ,  Jonathan Wong

Inventor： Lakshman R. Sehgal ,  Jonathan Wong

IPC: C12N15/11 ,  A61K39/235 ,  C12N15/861 ,  A61K31/715 ,  A01N63/00 ,  C07H21/04 ,  C12N15/86 ,  A61K38/44 ,  A61K39/00 ,  C07K14/745 ,  C12N7/00 ,  A61K48/00 ,  A61K38/00

CPC classification number: C12N15/86 ,  A61K38/00 ,  A61K38/44 ,  A61K39/001 ,  A61K48/00 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0075 ,  C07K14/7455 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2830/008 ,  C12Y113/11052

Abstract: A method for treating a renal disease in a subject is disclosed. The method includes administering into a kidney of the subject with an effective amount of a gutless adenoviral vector containing a polynucleotide encoding a therapeutic agent. The gutless adenoviral vector contains the nucleotide sequence of SEQ ID NO:13 or SEQ ID NO:15 and expresses the therapeutic agent in a kidney tissue of the subject.

公开(公告)号：US08420075B2

公开(公告)日：2013-04-16

申请号：US12750219

申请日：2010-03-30

Applicant: Lakshman R. Sehgal ,  Jonathan Wong

Inventor： Lakshman R. Sehgal ,  Jonathan Wong

IPC: A01N63/00 ,  A61K31/715 ,  A61K39/235 ,  C07H21/04 ,  C12N15/861

CPC classification number: C07K14/7455 ,  A61K38/00 ,  A61K48/00 ,  A61K48/005 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2799/022 ,  C12N2830/008

Abstract: The present invention relates to methods and compositions for treatment of cardiovascular and peripheral vascular diseases using ex vivo and in vivo gene delivery technologies. One aspect of the present invention relates to a method for treating a vascular disease by introducing a DNA sequence encoding a TM protein or its variant into a segment of a blood vessel in vivo using a gutless adenovirus vector. Another aspect of the present invention is to provide a method to deliver a gutless adenovirus vector carrying a DNA sequence encoding a TM protein or its variant using a stent.

公开(公告)号：US20110307076A1

公开(公告)日：2011-12-15

申请号：US13180378

申请日：2011-07-11

Applicant: Lakshman R. SEHGAL ,  Jonathan Wong

Inventor： Lakshman R. SEHGAL ,  Jonathan Wong

IPC: A61F2/04 ,  A61M25/10 ,  A61P9/10 ,  A61P9/12 ,  A61K31/711 ,  A61P13/12

CPC classification number: C12N15/86 ,  A61K38/00 ,  A61K38/44 ,  A61K39/001 ,  A61K48/00 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0075 ,  C07K14/7455 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2830/008 ,  C12Y113/11052

Abstract: A method for treating a renal disease in a subject is disclosed. The method includes administering into a kidney of the subject with an effective amount of a gutless adenoviral vector containing a polynucleotide encoding a therapeutic agent. The gutless adenoviral vector contains the nucleotide sequence of SEQ ID NO:13 or SEQ ID NO:15 and expresses the therapeutic agent in a kidney tissue of the subject.

公开(公告)号：US20100240740A1

公开(公告)日：2010-09-23

申请号：US12750219

申请日：2010-03-30

Applicant: Lakshman R. SEHGAL ,  Jonathan WONG

Inventor： Lakshman R. SEHGAL ,  Jonathan WONG

IPC: A61K48/00 ,  C12N15/63 ,  A61P9/00

CPC classification number: C07K14/7455 ,  A61K38/00 ,  A61K48/00 ,  A61K48/005 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2799/022 ,  C12N2830/008

Abstract: The present invention relates to methods and compositions for treatment of cardiovascular and peripheral vascular diseases using ex vivo and in vivo gene delivery technologies. One aspect of the present invention relates to a method for treating a vascular disease by introducing a DNA sequence encoding a TM protein or its variant into a segment of a blood vessel in vivo using a gutless adenovirus vector. Another aspect of the present invention is to provide a method to deliver a gutless adenovirus vector carrying a DNA sequence encoding a TM protein or its variant using a stent.

Abstract translation: 本发明涉及使用离体和体内基因递送技术治疗心血管和外周血管疾病的方法和组合物。 本发明的一个方面涉及通过使用无毒腺病毒载体将编码TM蛋白或其变体的DNA序列在体内的血管片段中引入来治疗血管疾病的方法。 本发明的另一方面是提供一种使用支架递送携带编码TM蛋白或其变体的DNA序列的无毒腺病毒载体的方法。

公开(公告)号：US06914127B2

公开(公告)日：2005-07-05

申请号：US10348579

申请日：2003-01-21

Applicant: Lakshman R. Sehgal ,  Richard E. De Woskin ,  Gerald S. Moss ,  Steven A. Gould ,  Arthur L. Rosen ,  Hansa Sehgal

Inventor： Lakshman R. Sehgal ,  Richard E. De Woskin ,  Gerald S. Moss ,  Steven A. Gould ,  Arthur L. Rosen ,  Hansa Sehgal

IPC: A61K38/00 ,  C07K14/805 ,  C08H1/00 ,  A61K37/14

CPC classification number: C08H1/00 ,  A61K38/00 ,  C07K14/805

Abstract: An acellular red blood cell substitute which comprises an essentially tetramer-free, substantially stroma-free, cross-linked, polymerized, pyridoxylated hemoglobin and a nontoxic, pharmaceutically acceptable carrier, its use and a process for preparing said acellular red blood cell substitute.

Abstract translation: 一种无细胞红细胞替代物，其包含基本上无四聚体，基本上无基质的交联聚合的吡哆醛化血红蛋白和无毒的药学上可接受的载体，其用途和制备所述无细胞红细胞替代物的方法。

公开(公告)号：US08877060B2

公开(公告)日：2014-11-04

申请号：US13285941

申请日：2011-10-31

Applicant: Lakshman R. Sehgal

Inventor： Lakshman R. Sehgal

IPC: B01D15/00 ,  C02F1/44 ,  A61L2/02 ,  A61M1/02 ,  A61L2/10 ,  A61L2/08

CPC classification number: A61M1/0281 ,  A61L2/022 ,  A61L2/088 ,  A61L2/10 ,  A61L2202/22 ,  A61M2202/0427 ,  B01D2315/16

Abstract: The present application relates to a method for removing pathogens from a transfusion grade platelet composition. The method comprises the steps of passing a platelet preparation through a first tangential flow filtration (TFF) device having a TFF filter, and collecting a retentate from the TFF device, wherein the retentate comprises filtered platelets to be used for transfusion. The platelet preparation comprises a platelet activation inhibitor and an anti-coagulant. During the TFF process, a diafiltration solution is added to the retentate to maintain the volume of the platelets.

Abstract translation: 本申请涉及从输血级血小板组合物中除去病原体的方法。 该方法包括以下步骤：使血小板制剂通过具有TFF过滤器的第一切向流过滤装置（TFF）装置，并从TFF装置收集滞留物，其中滞留物包括用于输血的过滤血小板。 血小板制剂包含血小板激活抑制剂和抗凝血剂。 在TFF过程中，将渗滤溶液加入到渗余物中以维持血小板的体积。

公开(公告)号：US08431334B2

公开(公告)日：2013-04-30

申请号：US12955200

申请日：2010-11-29

Applicant: Lakshman R. Sehgal

Inventor： Lakshman R. Sehgal

IPC: A01N1/02

CPC classification number: A01N1/0226 ,  A61M1/0272 ,  A61M1/0281 ,  A61M2202/0427

Abstract: The present invention relates to methods for preserving platelet. In one embodiment, the method includes the steps of mixing platelets with a platelet preservation composition to form a platelet preparation, storing the platelet preparation for a desired period of time, and removing the antiplatelet agent and the anticoagulant from the platelet preparation by diafiltration prior to transfusion of the platelets. The platelet preservation composition comprises an effective amount of an antiplatelet agent and an effective amount of an anticoagulant.

Abstract translation: 本发明涉及保留血小板的方法。 在一个实施方案中，该方法包括以下步骤：将血小板与血小板保存组合物混合以形成血小板制剂，将血小板制剂储存期望期限，并通过渗滤从血小板制剂中除去抗血小板剂和抗凝血剂， 输血血小板 血小板保存组合物包含有效量的抗血小板剂和有效量的抗凝血剂。

公开(公告)号：US08236300B2

公开(公告)日：2012-08-07

申请号：US13023322

申请日：2011-02-08

Applicant: Lakshman R. Sehgal ,  Jonathan Wong

Inventor： Lakshman R. Sehgal ,  Jonathan Wong

IPC: A01K63/00 ,  A61K39/325 ,  C07H21/04

CPC classification number: C12N15/86 ,  A61K38/00 ,  A61K38/44 ,  A61K39/001 ,  A61K48/00 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0075 ,  C07K14/7455 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2830/008 ,  C12Y113/11052

Abstract: A method for treating a renal disease in a subject is disclosed. The method includes administering into a kidney of the subject with an effective amount of a gutless adenoviral vector containing a polynucleotide encoding a therapeutic agent. The gutless adenoviral vector contains the nucleotide sequence of SEQ ID NO:13 or SEQ ID NO:15 and expresses the therapeutic agent in a kidney tissue of the subject.

Abstract translation: 公开了一种治疗受试者的肾脏疾病的方法。 该方法包括用有效量的含有编码治疗剂的多核苷酸的无毒腺病毒载体向受试者的肾中施用。 无毒腺病毒载体含有SEQ ID NO：13或SEQ ID NO：15的核苷酸序列，并在受试者的肾组织中表达治疗剂。

公开(公告)号：US20120157905A1

公开(公告)日：2012-06-21

申请号：US13290262

申请日：2011-11-07

Applicant: Lakshman R. SEHGAL

Inventor： Lakshman R. SEHGAL

IPC: A61M1/34 ,  B01D61/00

CPC classification number: B01D63/02 ,  A61M1/1698 ,  A61M1/36 ,  A61M2202/0014 ,  A61M2202/0233 ,  B01D19/0031 ,  B01D63/046 ,  B01D2313/20 ,  B01D2315/10

Abstract: The present application relates to a system and method for treating carbon monoxide poisoning. The system and method comprises passing the blood of a subject in need of such treatment through a filter unit comprising a gas permeable membrane to remove carbon monoxide from the blood by filtration against an extraction fluid enriched with oxygen and/or by exposure to one or more agents suitable for removing carbon monoxide or converting carbon monoxide to carbon dioxide, which is more readily exchangeable with oxygen compared to carbon monoxide.

Abstract translation: 本申请涉及一氧化碳中毒治疗的系统和方法。 该系统和方法包括使需要这种治疗的受试者的血液通过包括透气膜的过滤器单元，以通过过滤富含氧气的提取液和/或暴露于一种或多种来从血液中除去一氧化碳 适合于除去一氧化碳或将一氧化碳转化成二氧化碳的试剂，与一氧化碳相比，它更容易与氧气相交换。

公开(公告)号：US20110223662A1

公开(公告)日：2011-09-15

申请号：US13098085

申请日：2011-04-29

Applicant: Lakshman R. Sehgal

Inventor： Lakshman R. Sehgal

IPC: C12N5/02

CPC classification number: A01N1/0215 ,  A01N1/02 ,  A01N1/0226

Abstract: The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state.

Abstract translation: 本发明涉及用于储存血小板以保持血小板功能和新鲜度的组合物和方法。 更具体地，本发明涉及使用具有抗血小板剂，抗凝血剂和氧载体的防腐剂组合物，以保持血小板的新鲜度。 另外，组合物还可以含有超短效广谱抗微生物剂。 防腐剂组合物可用于将血小板置于液态，冷冻状态或冻干状态。