The present disclosure relates to certain polypeptides derived from prokaryotic DGT enzymes, and nucleic acids useful in encoding the same, for example, novel polypeptides involved in metabolism of N-(phosphonomethyl) glycine, and nucleic acids encoding such polypeptides. In some examples, such a polypeptide confers (or increases) tolerance to glyphosate in a plant cell wherein the polypeptide is heterologously expressed, for example, without adversely affecting the binding of EPSP synthase with its natural substrate, phosphoenolpyruvate (PEP).