Invention Grant
- Patent Title: Linked ligation
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Application No.: US16467870Application Date: 2017-12-07
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Publication No.: US11268137B2Publication Date: 2022-03-08
- Inventor: Andrea Marziali , Joel Pel
- Applicant: Boreal Genomics, Inc.
- Applicant Address: CA Vancouver
- Assignee: Boreal Genomics, Inc.
- Current Assignee: Boreal Genomics, Inc.
- Current Assignee Address: CA Vancouver
- Agency: Brown Rudnick LLP
- International Application: PCT/IB2017/057732 WO 20171207
- International Announcement: WO2018/104908 WO 20180614
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6827 ; C12Q1/6806 ; C12Q1/6855 ; C12Q1/686 ; C12Q1/6869

Abstract:
The invention generally relates to capturing, amplifying, and sequencing nucleic acids. In certain embodiments, copies of the sense and antisense strands of a duplex template nucleic acid are captured using linked capture probes and multiple binding and extension steps to improve specificity over traditional single binding target capture techniques. Methods of seeding sequencing clusters with sense and antisense strands of a target nucleic acid are also disclosed including identifying the strands using sense-specific barcodes and confirming base calls using two sense-specific sequencing reads. Linked adapters may be used to increase adapter ligation selectively or efficiency and yield.
Public/Granted literature
- US20190300942A1 LINKED LIGATION Public/Granted day:2019-10-03
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