The invention discloses high-strength steel with the fatigue stress amplitude equal to or larger than 400 MPa. The high-strength steel with the fatigue stress amplitude equal to or larger than 400 MPacomprises the components, by weight, 0.12% to 0.18% of C, 0.10% to 0.40% of Si, 1.20% to 1.60% of Mn, smaller than or equal to 0.015% of the sum of P and S, 0.10% to 0.45% of Cr, 0.42% to 0.75% of Mo, 0.05% to 0.20% of Ni, and 0.045% to 0.100% of the sum of Nb, Ti ,V and B. A method comprises the production steps of carrying out smelting as clean steel, then carrying out continuous casting to form blanks, and carrying out hot rolling on the blanks till the thickness of each blank reaches the finished product thickness; carrying out quenching; carrying out backfire; and carrying out natural cooling to the room temperature. According to the high-strength steel with the fatigue stress amplitude equal to or larger than 400 MPa and the method, on the conditions that the yield strength ranges from 900 MPa to 1, 000 MPa, the tensile strength is larger than or equal to 1, 000 MPa, and the elogation A is larger than or equal to 15%, the microstructure is finer through microalloy control, and the fatigue stress limit of the steel is larger than 400 MPa which is at least 20 MPa higher than 380 MPa as required by the present requirements. No breaking happens to the high-strength steel with the fatigue stress amplitude equal to or larger than 400 MPa after a common fatigue test is carried out, and a new window is opened for the fatigue life design of an existing component.