The invention discloses application of the human PYCR1 gene and related drugs. The invention discloses application of the human PYCR1 gene to tumor treatment, tumor diagnosis and drug preparation. The invention further discloses human PYCR1 gene micromolecule interference RNA, a human PYCR1 gene interference nucleic acid constructor, a human PYCR1 gene interference slow virus, and application thereof. The siRNA or the nucleic acid constructor and slow virus comprising the siRNA sequence can specifically inhibit the expression of the human PYCR1 gene, particularly, the slow virus can efficiently infect target cells, efficiently inhibit the expression of the PYCR1 gene in target cells so as to inhibit the growth of tumor cells and promote apoptosis of tumor cells, and thus having great significance in tumor treatment.