The invention discloses an expression vector of a beta-glucosidase mutant protein. The expression vector is constructed with the method comprising the following steps: (1) cloning a DNA sequence of the beta-glucosidase mutant protein; (2) cloning a DNA sequence of a photosensitive regulation unit; (3) linking DNA restriction fragments of the beta-glucosidase mutant protein and DNA restriction fragments of the photosensitive regulation unit, which are obtained through digestion, to a pET22b(+) vector to obtain the expression vector of the beta-glucosidase mutant protein. The photo-inducible expression vector is constructed by inserting the photosensitive regulation unit and can perform induced expression on the beta-glucosidase mutant protein with an illumination means, compared with the traditional induced expression method, the light induction method has the characteristics of being high in expression quantity and free of exogenous additives, and besides, the protein expression intensity of the light induction method can be controlled through illumination intensity without affecting a follow-up purification step.