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Invention Publication
CN104988212A 强冬性白菜型冬油菜CAT酶分子标记及QTL定位 有权

强冬性白菜型冬油菜CAT酶分子标记及QTL定位
Abstract:
本发明公开了强冬性白菜型冬油菜CAT酶分子标记及QTL位点，其筛选步骤包括：（1）利用多代套袋自交的亲本强冬性超强抗寒冬油菜‘陇油7号’和强抗寒冬油菜‘陇油9号’杂交构建F2代分离群体；（2）采用CTAB法提取两亲本及F2代分离群体的叶片DNA；（3）利用SSR和InDel引物对两亲本DNA进行多态性引物筛选；（4）通过F2分离群体获得基因型数据构建遗传连锁图和QTL分析；（5）获得了3个强冬性白菜型冬油菜CAT酶的QTL位点，分别命名为Qcat.gsau-2A-1、Qcat.gsau-2A-2和Qcat.gsau-2A-3；3个与强冬性白菜型冬油菜CAT酶QTL位点连锁的分子标记BrID90127、BrID10421和BrID10709。
Abstract(English):

The invention discloses strong winterness brassica campestris L. CAT enzyme molecule markers and QTL loci. The screening steps are that (1), multi-generation bagging selfing parent strong winterness superstrong cold resistant winter rape 'the 7th Longyou' and strong cold resistant winter rape 'the 9th Longyou' are utilized for hybridization to construct a generation F2 segregation population; (2), a CTAB method is utilized for extracting leaf DNA of the generation F2 segregation population and the two parents; (3), SSR and InDel primers are utilized for performing polymorphism primer selection on the DNA of the two parents; (4), genetype data are obtained through the generation F2 segretation population to construct a genetic linkage map and QTL analysis; and (5), three strong winterness brassica campestris L. CAT enzyme QTL loci are obtained and named as Qcat.gsau-2A-1, Qcat.gsau-2A-2 and Qcat.gsau-2A-3 respectively; and three molecule markers linked with the strong winterness brassica campestris L. CAT enzyme QTL loci are BrID90127,BrID10421 and BrID10709.

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