The invention discloses an Actinomyces Israeli production technology, an Actinomyces Israeli preparation and an Actinomyces Israeli production apparatus. The production technology adopts a liquid-solid biphasic cultivation method to realize the mass production of Actinomyces Israeli, liquid fermentation is carried out to obtain a large amount of mycelia and blastospores and a small amount of conidiospores, and the above obtained substances are transferred to a solid cultivation medium to generate a large amount of aerial conidiospores. An Actinomyces Israeli pill or pulvis prepared through the technology is convenient to store, and can be dissolved in a 0.1M sodium citrate solution or is diluted by adding water when the pill or pulvis is used. The production apparatus comprises a disc body, the base plate of the disc body is punched with a plurality of holes, and at least one metal screen covering all the holes is fixed on the base plate. The apparatus guarantees the oxygen and humidity required by the solid cultivation of the Actinomyces Israeli, effectively prevents the living contaminants, and substantially improves the sporulation quantity of the Actinomyces Israeli.