The invention discloses a streptomycete strain and an application thereof in the production of actinomycin. The provided strain is streptomycete MS449 with a collection number of CGMCC No. 5452. When the streptomycete MS449 is not optimized by using any culture medium or fermentation condition, the actinomycin X2, actinomycin D and actinomycin X0beta yields of the streptomycete MS449 can respectively reach 1.92, 1.77 and 0.15mg/ml, which are all higher than currently reported actinomycin yields. The invention also provides a production method of actinomycin. According to the invention, actinomycin is produced through the fermentation of streptomycete MS449. The fermentation is stable, the compound separation technology is simple, and the yield is high. The strain provided by the inventionhas excellent industrialization potential and prospect.