The invention discloses a cucumber violaxanthin decyclization oxidase gene promoter and application thereof. The promoter is any one of the following 1)-3): 1) a DNA molecule shown as a sequence 1 in a sequence table; 2) a DNA molecule which is hybridized with the DNA sequence limited in the 1) under strict conditions and has promoter function; and 3) a DNA molecule which has over 90 percent of homology with the DNA sequence limited in the 1) or the 2) and has promoter function. The invention discloses a sequence of the cucumber violaxanthin decyclization oxidase gene promoter. Proved by experiments, the promoter has efficient and specific expression in a green plant tissue, mainly in the green plant tissue for photosynthesis, such as expression for promoting a target gene in a leaf, so the promoter has significance for further researching the biological information of related molecules of plant photosynthesis.