The invention relates to a wolfberry seed oil self-microemulsifying nano composition characterized by comprising the following components in percentage by weight: 0.1-15 percent of wolfberry seed oil, 0-20 percent of oil phase, 0.05-90 percent of surfactant, 0-40 percent of aid surfactant and the balance of water. The preparation method of the wolfberry seed oil self-microemulsifying nano composition comprises the following steps of: uniformly mixing the wolfberry seed oil, the oil phase, the surfactant and the aid surfactant according to a prescription proportion; directly packaging into soft capsules or adding other materials to prepare into corresponding solid, semi-solid or liquid products.