A method and apparatus are provided for transmitting and receiving Uplink Control Information (UCI) in a wireless communication system. The method includes generating the UCI including at least one of channel quality indicator (CQI) information and hybrid automatic repeat request (HARQ) information; identifying, if the terminal is configured with a plurality of serving cells, a serving cell among the plurality of serving cells based on information on a cell index of the serving cell; and transmitting the UCI on a physical uplink shared channel (PUSCH) of the identified serving cell.