Described is a hemoglobin and hematocrit analyzer, and an analyzing method thereof. The method includes: scanning and taking an image signal of the palpebral conjunctiva of a subject by a scanning unit; receiving the image signal by an analyzing unit connected to the scanning unit; providing a default colorimetric scale by a database connected to the analyzing unit; inputting a clinical test result into the analyzing unit through an input unit connected to the analyzing unit. The image signal is transformed by the analyzing unit to a measured color value. The measured color value is compared with the default colorimetric scale to obtain a test result. The measured color value and the clinical test result are provided as feedback to the database.