Invention Grant
- Patent Title: Method for detection of Legionella bacteria employing purified antigen-specific antibodies
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Application No.: US14828823Application Date: 2015-08-18
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Publication No.: US09989529B2Publication Date: 2018-06-05
- Inventor: Vladimir A. Koulchin , Norman J. Moore , Elena V. Molokova
- Applicant: Alere Scarborough, Inc.
- Applicant Address: US ME Scarborough
- Assignee: Alere Scarborough, Inc.
- Current Assignee: Alere Scarborough, Inc.
- Current Assignee Address: US ME Scarborough
- Agency: Foley Hoag LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/569 ; C07K16/12 ; G01N33/53 ; G01N21/78 ; G01N21/75 ; G01N21/77

Abstract:
The present invention involves extracting from Legionella bacteria, particularly L. pneumophila bacteria, an essentially protein-free O-polysaccharide or carbohydrate antigen, coupling this antigen to an activated chromatographic column through a protein space molecule which is first conjugated to the antigen, utilizing the column thus prepared for the affinity purification of raw polyvalent antibodies to the same Legionella bacterium from which the O-polysaccharide or carbohydrate antigen was separated—thereby obtaining antigen-specific antibodies which are useful for the rapid detection of the corresponding Legionella bacterium or its antibody in human bodily fluids such as urine, sputum, blood and the like or in environmental samples suspected of harboring theLegionella bacterium.
Public/Granted literature
- US20160047807A1 Method for Detection of Legionella Bacteria Employing Purified Antigen-Specific Antibodies Public/Granted day:2016-02-18
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