The present invention provides a display substrate including a plurality of pixel units. Wherein, the plurality of pixel units include a plurality of oblique pixel units, and length directions of the plurality of oblique pixel units are oblique relative to a horizontal direction. The present invention further provides a display panel and a stereoscopic display device. When stereoscopic display is performed using the display panel according to the present invention, the resolutions of each parallax image in both a length direction and a width direction of the display panel decrease, i.e., the problem that the resolution of a single-eye image decreasing only in one direction when stereoscopic display is performed is avoided, thereby improving the display effect.