The present disclosure provides a driving signal generating circuit, a driving signal generating method and a 3D display device. The driving signal generating circuit includes a voltage conversion unit, an output unit and a control unit. The voltage conversion unit is configured to convert an external input voltage into a plurality of polarization voltages for driving a polarization control panel in an active polarization 3D display device, and transmit the plurality of polarization voltages to the output unit. The control unit is configured to generate a control signal. The output unit is configured to output one of the polarization voltages or a zero voltage to the polarization control panel in a time-division manner according to the control signal.