Toolstring topology mapping systems having a downhole toolstring including a master node controller and a plurality of nodes and related methods. The methods involve querying each of the plurality of nodes in the downhole toolstring for actual topology information, receiving actual topology information from each of the plurality of nodes in the downhole toolstring in response to querying, and generating a topology map of the downhole toolstring based on the actual topology information from each of the plurality of nodes in the downhole toolstring. The methods can also involve comparing the topology map with the input topology information to identify topology mismatch and then take remedial actions if needed.