A semiconductor device includes a sense amplification unit suitable for sensing and amplifying data loaded on a data line pair; a pull-up driving unit suitable for supplying a first voltage to a pull-up power line of the sense amplification unit as a pull-up driving voltage in an active mode, and supplying second voltage higher than the first voltage to the pull-up power line as the pull-up driving voltage during an initial period of a precharge mode; a pull-down driving unit suitable for supplying a third voltage to a pull-down power line of the sense amplification unit as a pull-down driving voltage during the active mode and the initial period of the precharge mode; and a post over-driving control unit suitable for adjusting the initial period of the precharge mode by detecting a voltage level of the pull-up power line.