Systems and methods herein utilize psychoacoustic principles to process audio signals to achieve a desired result. In some embodiments, an exemplary process utilizes a combination of re-harmonization and auditory processing techniques in which a plurality of filtered audio cues are sampled, created and down-mixed into a final music file output, which may be designed to trigger specific effects on the function of the body and the brain. In some embodiments, enhanced audio material may be altered during the stream or fixed for a desired result at the beginning of the stream. In some embodiments, a signal enhancement process may be embedded into silicon or a media player, decoder or device with software. In other embodiments, audio or video material may be encoded or finalized into the media file.