The embodiment of the present disclosure provides a pixel unit driving circuit comprising: a driving unit; a charging unit; a storage unit configured to be charged during the charging stage of the pixel unit driving circuit, and provide a control voltage to the driving unit during a driving stage of the pixel unit driving circuit; a lighting control unit configured to make that a driving current provided from the driving unit to the lighting element during the driving stage of the pixel unit driving circuit is independent on a threshold voltage of the driving unit; and the driving control unit connected to the lighting control unit, the storage unit and the driving unit and configured to control the supply of the control voltage of the driving unit. According the embodiments of the present disclosure, the influence of the threshold voltage of the driving unit on the operating current is eliminated by providing the lighting control unit and the driving control unit, so as to moderate the drift of the threshold voltage caused by process procedure and a long term operation and to ensure the uniformity of the displayed brightness of the lighting element.