A chromatographic medium having a separating agent layer, which is used to separate target substances, and a permeation layer, which is laminated so as to face the separating agent layer and which is used to enable permeation of the target substances separated by the separating agent layer, wherein a region in which the permeation layer is not laminated is present on a part of the separating agent layer, the separating agent layer exhibits a separating property for the target substances and exhibits an optical responsiveness to ultraviolet rays, and the permeation layer exhibits an optical responsiveness that is different from those of the target substances and the separating agent layer.