The present invention relates to apparatus for in real time detecting biological particles and non-biological particles in the atmosphere, the apparatus comprising: an impactor adapted to sort the biological particles and non-biological particles absorbed from the outside by size; a charger adapted to charge the biological particles and non-biological particles sorted by means of the impactor to specific charge (positive or negative charge); a separator adapted to introduce the biological particles and non-biological particles charged by the charger thereinto and to sort the biological particles and non-biological particles charged to different charge quantities from each other; and a particle measurement sensor adapted to measure the concentrations of the particles discharged from an outlet.