Invention Grant
- Patent Title: Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme
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Application No.: US14199569Application Date: 2014-03-06
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Publication No.: US09695461B2Publication Date: 2017-07-04
- Inventor: Petra Siegert , Stefan Evers , Marion Merkel , Nina Mussmann , Hendrik Hellmuth , Timothy O'Connell , Karl-Heinz Maurer , Ulrich Schwaneberg , Felix Jakob , Ronny Martinez , Brian Laufs , Ayhan Aydemir , Thomas Weber
- Applicant: Henkel AG & Co. KGaA
- Applicant Address: DE Duesseldorf
- Assignee: Henkel AG & Co. KGaA
- Current Assignee: Henkel AG & Co. KGaA
- Current Assignee Address: DE Duesseldorf
- Agent Thomas G. Krivulka
- Priority: DE102011118027 20110912
- Main IPC: A01N25/30
- IPC: A01N25/30 ; C12N9/54 ; C12Q1/37 ; C11D3/386 ; C12N9/96

Abstract:
The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.
Public/Granted literature
- US20140186868A1 METHOD FOR ADAPTING A HYDROLYTIC ENZYME TO A COMPONENT THAT STABILIZES THE HYDROLYTIC ENZYME Public/Granted day:2014-07-03
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