Embodiments of the invention disclose an organic light-emitting diode array substrate and a manufacturing method thereof, and a display device. The array substrate comprises: a base substrate, a thin film transistor disposed above the base substrate, an organic light-emitting diode and a filling layer, the organic light-emitting diode including a first electrode, a second electrode, and an organic light-emitting layer disposed between the first electrode and the second electrode, wherein, in a light transmissive region of the organic light-emitting diode array substrate, the base substrate, the filling layer and the organic light-emitting layer of the organic light-emitting diode are disposed to be sequentially abutting.