A method and system for transferring real-time audio/video stream (AVS) is provided. The method for transferring real-time AVS includes the following steps of: first, receiving a real-time AVS by a server; transforming the real-time AVS into a fragmented media data (FMD) and storing the FMD in a storage module of the server, wherein the FMD comprises a starting-point, an overall fragment information and at least one media fragment (MF); next, generating a segmented media data (SMD) by encapsulating the FMD when the server receives a playback command. The step of generating the SMD comprises encapsulating the starting-point and the overall fragment information into an initial segment information of the SMD. Each playable segment of the SMD has multiple MFs and a segment index recording segment order to indicate each MF. The final step is to transfer the SMD to browser software for playback.