The present invention provides a manufacturing method of a membrane electrode assembly which makes it possible to produce a polymer electrolyte fuel cell at a high level of productivity. According to the present invention, it is possible to make differences in characteristics between a first catalyst electrode 11A and a second catalyst electrode 11B, which are formed on the both surfaces of a polymer electrolyte membrane 3, without changing materials of the first and the second catalyst electrode 11A and 11B. In the present invention, the first and the second catalyst electrode 11A and 11B are adhered to the polymer electrolyte membrane 3 by sticking the first catalyst electrode 11A by a first roll press 7 followed by sticking the second catalyst electrode 11B by a second roll press 8.