The present invention discloses a microphone detection circuit for detecting whether an external device includes a microphone. The microphone detection circuit comprises: an audio circuit for receiving an analog input signal which is a signal from the external device or a preset signal. The audio circuit comprises: an analog-to-digital conversion unit to generate a digital audio signal according to the analog input signal; a decision unit, coupled to the analog-to-digital conversion unit, to determine whether the digital audio signal satisfies a predetermined threshold to thereby generate an analysis result, wherein if the digital audio signal satisfies the predetermined threshold, the analysis result indicates that the external device includes a microphone; and a control unit, coupled to the decision unit, to control an operation of the audio circuit according to the analysis result.