Invention Grant
- Patent Title: Detection method for cervical HPVS
- Patent Title (中): 宫颈HPVS检测方法
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Application No.: US12741018Application Date: 2007-11-01
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Publication No.: US09435001B2Publication Date: 2016-09-06
- Inventor: Christophorus Joannes Lambertus Maria Meijer , Petrus Josephus Ferdinandus Snijders
- Applicant: Christophorus Joannes Lambertus Maria Meijer , Petrus Josephus Ferdinandus Snijders
- Applicant Address: NL Amsterdam
- Assignee: SELF-SCREEN B.V.
- Current Assignee: SELF-SCREEN B.V.
- Current Assignee Address: NL Amsterdam
- Agency: Morrison & Foerster LLP
- International Application: PCT/NL2007/050526 WO 20071101
- International Announcement: WO2009/057993 WO 20090507
- Main IPC: C12Q1/70
- IPC: C12Q1/70 ; C07H21/04 ; A61K45/06

Abstract:
The invention describes a consensus PCR based method (i.e. HRE7-PCR) for the simultaneous detection of 14 Human Papilloma Virus types (i.e. HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are classified as (probably) high-risk, relating to the causation of cervical cancer) and a candidate hrHPV type (i.e. HPV 67) using sets of 6 overlapping forward primers and 9 overlapping backward primers that together amplify a fragment of about (215) to (245) base pairs of the E7 open reading frame of these hrHPV types. For the detection of reaction products an EIA format can be used with the aid of a cocktail of type-specific oligoprobes as exemplified herein. Furthermore, we have developed a method for an efficient typing of these (15) HPVs that is compatible with the method for detection. This RLB typing system involves hybridization of PCR products with immobilized type-specific oligoprobes.
Public/Granted literature
- US20100304362A1 NEW DETECTION METHOD FOR CERVICAL HPVS Public/Granted day:2010-12-02
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