Invention Grant
US09410148B2 Method for constructing libraries of non-standard peptide compounds comprising N-methyl amino acids and other special (non-standard) amino acids and method for searching and identifying active species
有权
构建包含N-甲基氨基酸和其他特殊（非标准）氨基酸的非标准肽化合物的文库的方法和用于检测和鉴定活性物质的方法
- Patent Title: Method for constructing libraries of non-standard peptide compounds comprising N-methyl amino acids and other special (non-standard) amino acids and method for searching and identifying active species
- Patent Title (中): 构建包含N-甲基氨基酸和其他特殊（非标准）氨基酸的非标准肽化合物的文库的方法和用于检测和鉴定活性物质的方法
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Application No.: US13821940Application Date: 2011-09-08
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Publication No.: US09410148B2Publication Date: 2016-08-09
- Inventor: Hiroaki Suga , Yusuke Yamagishi
- Applicant: Hiroaki Suga , Yusuke Yamagishi
- Applicant Address: JP Tokyo
- Assignee: THE UNIVERSITY OF TOKYO
- Current Assignee: THE UNIVERSITY OF TOKYO
- Current Assignee Address: JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2010-202012 20100909
- International Application: PCT/JP2011/070439 WO 20110908
- International Announcement: WO2012/033154 WO 20120315
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C40B40/10 ; C40B30/04 ; C40B50/06 ; C07K2/00 ; C12P21/02 ; C40B20/04 ; C12N15/67
Abstract:
A method for screening a non-standard peptide compound in the peptide library that binds to the target substance, comprising the steps: (i) preparing a non-standard peptide library wherein a special (non-standard) amino acid is randomly incorporated into the peptide sequence by a cell-free (in vitro) translation system comprising a tRNA acylated by a special (non-standard) amino acid; (ii) bringing the obtained peptide library in contact with a target substance; and (iii) selecting a non-standard peptide that binds to the target substance as an active peptide.
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