Invention Grant
US09409971B2 Method of producing recombinant high molecular weight vWF in cell culture
有权
在细胞培养中生产重组高分子量vWF的方法
- Patent Title: Method of producing recombinant high molecular weight vWF in cell culture
- Patent Title (中): 在细胞培养中生产重组高分子量vWF的方法
-
Application No.: US14339319Application Date: 2014-07-23
-
Publication No.: US09409971B2Publication Date: 2016-08-09
- Inventor: Leopold Grillberger , Manfred Reiter , Wolfgang Mundt
- Applicant: Baxalta Incorporated , Baxalta GmbH
- Applicant Address: US IL Bannockburn unknown Glattpark (Opfikon)
- Assignee: Baxalta Incorporated,Baxalta GmbH
- Current Assignee: Baxalta Incorporated,Baxalta GmbH
- Current Assignee Address: US IL Bannockburn unknown Glattpark (Opfikon)
- Agency: Morgan, Lewis & Bockius LLP
- Main IPC: C12P21/04
- IPC: C12P21/04 ; C07K14/755 ; C12N9/64 ; A61K38/00
Abstract:
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimeric WF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
Public/Granted literature
- US20150018525A1 METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT vWF IN CELL CULTURE Public/Granted day:2015-01-15
Information query
