An image pickup apparatus includes: first and second photoelectric conversion units that are disposed in a semiconductor substrate, are configured to accumulate charges, and are of a first conductivity type; an electrode that is disposed on a region between the first and second photoelectric conversion units and is configured to control an electrical conduction between the first and second photoelectric conversion units; a first semiconductor region that is formed under the electrode and between the first and second photoelectric conversion units, is continuous from the first photoelectric conversion unit to the second photoelectric conversion unit, and is of the first conductivity type; and a second semiconductor region that is formed under the electrode and between the first and second photoelectric conversion units, is provided at a depth different from that of the first semiconductor region, and is of a second conductivity type.