Invention Grant
US09382581B2 Primers with modified phosphate and base in allele-specific PCR
有权
在等位基因特异性PCR中具有修饰的磷酸酯和碱基的引物
- Patent Title: Primers with modified phosphate and base in allele-specific PCR
- Patent Title (中): 在等位基因特异性PCR中具有修饰的磷酸酯和碱基的引物
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Application No.: US14092133Application Date: 2013-11-27
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Publication No.: US09382581B2Publication Date: 2016-07-05
- Inventor: Stefanie Froehner , Dieter Heindl , Dirk Kessler , Nancy Schoenbrunner , Alison Tsan
- Applicant: Roche Molecular Systems, Inc.
- Applicant Address: US CA Pleasanton
- Assignee: ROCHE MOLECULAR SYSTEMS, INC.
- Current Assignee: ROCHE MOLECULAR SYSTEMS, INC.
- Current Assignee Address: US CA Pleasanton
- Agent David J Chang
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating both a nucleotide with a base covalently modified at the exocyclic amino group and a modified phosphate.
Public/Granted literature
- US20140170650A1 Primers with Modified Phosphate and Base in Allele-Specific PCR Public/Granted day:2014-06-19
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