There is provided a content orchestration system and a method for use by the content orchestration system to enable assembly of one or more customized content streams. In one implementation, such a content orchestration system includes a computing platform having a processor, and a content orchestration engine for execution by the processor. The content orchestration engine is configured to receive data describing multiple content sources capable of providing content for use in assembling a customized content stream. The content orchestration engine is also configured to identify customization guidelines corresponding to a destination of the customized content stream, and to determine instructions for assembling the customized content stream based on the data and the customization guidelines.