An image sensor having image sensor pixels operable in multiple gain modes is provided. An image sensor pixel may include a photodiode, a charge transfer transistor, a reset transistor, a source follower (SF) transistor, a row select transistor, and a capacitor that is directly connected across the gate terminal and a selected source-drain terminal of the SF transistor. The SF transistor and the row select transistor may be selectively coupled to a power supply line and a column output line by asserting either a first control signal or a second control signal. The first control signal is asserted to place the pixel in a low conversion gain mode, whereas the second control signal is asserted to place the pixel in a high conversion gain mode. The charge transfer transistor may always be activated during the low conversion gain mode and may be selectively activated during the high conversion gain mode.