The use of SIRT7 (sirtuin 7) as a marker for diagnosis of liver cancer is described. The disclosure variously relates to a liver cancer diagnostic marker including SIRT7 gene, a liver cancer diagnostic composition, a kit and microarray including the same, and a method of diagnosing liver cancer using the same. Also described is a method for screening a substance capable of treating liver cancer by inhibiting the expression of SIRT7 gene or protein, and a composition for preventing or treating liver cancer, which includes such substance. The disclosure further relates to the use of SIRT7 gene as a cancer diagnostic marker and the anticancer use of inhibition of SIRT7 expression, as well as the use of the specific miRNA to regulate the cell cycle and inhibit tumor growth by the expression of SIRT7 gene.