Invention Grant
US09249456B2 Base specific cleavage of methylation-specific amplification products in combination with mass analysis
有权
甲基化特异性扩增产物的碱基特异性切割与质量分析相结合
- Patent Title: Base specific cleavage of methylation-specific amplification products in combination with mass analysis
- Patent Title (中): 甲基化特异性扩增产物的碱基特异性切割与质量分析相结合
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Application No.: US11089805Application Date: 2005-03-24
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Publication No.: US09249456B2Publication Date: 2016-02-02
- Inventor: Mathias Ehrich , Dirk Johannes Van Den Boom
- Applicant: Mathias Ehrich , Dirk Johannes Van Den Boom
- Applicant Address: US CA San Diego
- Assignee: AGENA BIOSCIENCE, INC.
- Current Assignee: AGENA BIOSCIENCE, INC.
- Current Assignee Address: US CA San Diego
- Agency: Grant IP, Inc.
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
Methods, combinations and kits are provided for identifying the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or for identifying the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule. Methylation state identification is performed by treating a methylated target nucleic acid molecule with a reagent that modifies one or more nucleotides in the target nucleic acid molecule as a function of the methylation state of the target nucleic acid molecule, methylation specifically amplifying treated target nucleic acid molecule, fragmenting amplified products, and detecting one or more fragments to thereby identify the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule.
Public/Granted literature
- US20050272070A1 Base specific cleavage of methylation-specific amplification products in combination with mass analysis Public/Granted day:2005-12-08
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