A physiological detection module includes a conductive transmission unit, a coupler, and an electrical connector. The coupler is arranged on a surface of the conductive transmission unit. The coupler includes a receiving chamber extending therethrough. The coupler includes a positioning groove circumferentially formed thereon for coupling and positioning. The electrical connector extends through the conductive transmission unit and the receiving chamber of the coupler to connect the conductive transmission unit and the coupler together. As such, a physiological detection module is provided, which can be manufactured separately, is easy to manufacture, can be efficiently assembled for use, is securely coupled, and can prevent short-circuiting.