A method and an apparatus for transmitting and receiving a signal using interference alignment in a multi-cellular network are provided. The method includes determining a total number Sj of effective Inter-Cell Interference (ICI) channels of a j-th cell, determining a number tj,i of effective ICI channels caused by each of other Base Stations (BSs) and a number nj,i(s) of ICI channels aligned to each effective ICI channel, acquiring information about a channel matrix between users of the j-th cell and each BS, determining effective ICI channels reaching from the other BSs to users of the j-th cell and a receive beamforming matrix by reference to tj,i, nj,i(s) and information about the channel matrix, transmitting feedback information indicating the channel matrix and the receive beamforming matrix to the BS of the j-th cell, and receiving a signal from the BS of the j-th cell using the determined receive beamforming matrix.