An imaging optical system includes a first optical unit having positive refractive power making an image at an image plane at an enlargement-side of the imaging optical system and an intermediate image at an intermediate image position in the imaging optical system conjugate to each other, and a second optical unit having positive refractive power making the intermediate image and an image at an image plane at a reduction-side of the imaging optical system conjugate to each other, wherein, when a focal length of the first optical unit is denoted by fF and a focal length of the second optical unit is denoted by fR, the following condition is satisfied: 0