Invention Grant
- Patent Title: Detection and quantification of hydroxymethylated nucleotides in a polynucleotide preparation
- Patent Title (中): 多核苷酸制备中羟甲基化核苷酸的检测和定量
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Application No.: US13392286Application Date: 2010-08-25
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Publication No.: US09034597B2Publication Date: 2015-05-19
- Inventor: Jurate Bitinaite , Romualdas Vaisvila , Sriharsa Pradhan , Yu Zheng , Richard J. Roberts , Devora Cohen-Karni , Christopher Noren , Elisabeth A. Raleigh , Geoffrey Wilson , Hang-Gyeong Chin
- Applicant: Jurate Bitinaite , Romualdas Vaisvila , Sriharsa Pradhan , Yu Zheng , Richard J. Roberts , Devora Cohen-Karni , Christopher Noren , Elisabeth A. Raleigh , Geoffrey Wilson , Hang-Gyeong Chin
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agency: New England Biolabs, Inc
- Agent Harriet M. Strimpel
- International Application: PCT/US2010/046632 WO 20100825
- International Announcement: WO2011/025819 WO 20110303
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.
Public/Granted literature
- US20120156677A1 Detection and Quantification of Hydroxymethylated Nucleotides in a Polynucleotide Preparation Public/Granted day:2012-06-21
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