Invention Grant
- Patent Title: Magnetic separation of cells
- Patent Title (中): 细胞的磁分离
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Application No.: US13849124Application Date: 2013-03-22
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Publication No.: US09034589B2Publication Date: 2015-05-19
- Inventor: Thomas Boland , Sylvia L. Natividad , Julio Rincon
- Applicant: Thomas Boland , Sylvia L. Natividad , Julio Rincon
- Applicant Address: US TX Austin
- Assignee: Board of Regents, The University of Texas System
- Current Assignee: Board of Regents, The University of Texas System
- Current Assignee Address: US TX Austin
- Agency: Ortiz & Lopez, PLLC
- Agent Luis M. Ortiz; Kermit D. Lopez
- Main IPC: G01N33/53
- IPC: G01N33/53 ; G01N33/555 ; G01N33/569

Abstract:
An apparatus, system, and method for magnetic separation of cells are disclosed. By combining inkjet printing technology and magnetic labeling of cells, accurate cell counts are obtained using an optical microscope. Mouse CD4+ lymphocytes are attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer. The labeled cells are then attached to a glass slide covering a permanent magnet. Cell counts can be obtained by use of regular and inverted optical microscopes and imaging software. The magnetically-labeled beads are collected for evaluation on a modified polymer coupon that is placed in front of a permanent magnet and the unlabeled cells fall into an excess container. Flow cytometry results verify the presence of the CD4+ protein on the LBRM-33 lymphocytes membrane. Protein-specific attachment of magnetic microspheres to the lymphocytes is utilized for sorting CD4+ lymphocytes.
Public/Granted literature
- US20140287437A1 MAGNETIC SEPARATION OF CELLS Public/Granted day:2014-09-25
Information query
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