Some embodiments herein disclose a method in a second node (12,13) for performing a positioning measurement on at least uplink signals transmitted by a wireless device (10) served by a first node (12) in a wireless communication network (1). The second node (12,13) obtains information related to a non-contiguous uplink configuration associated with a carrier frequency, wherein the non-contiguous uplink configuration further comprises one or more multi-cluster uplink transmissions from the wireless device (10). Furthermore, the second node (12,13) performs a positioning measurement on at least signals transmitted by the wireless device (10) in the one or more multi-cluster transmissions determined based on the obtained information.