A secondary side synchronous rectification control circuit is disclosed. The control circuit includes an inverted amplifier, a first comparator, and a driving unit. The inverted amplifier has an input end for receiving a drain source voltage signal from a synchronous rectification transistor and outputting an inverted amplification signal. The first comparator receives the inverted amplification signal and a first reference voltage for outputting a first comparison signal. The driving unit receives the first comparison signal and generates a driving signal according to the first comparison signal, for controlling the conduction status of the synchronous rectification transistor. The drain source voltage of the synchronous rectification transistor in the present invention is inverted amplified by an inverted amplifier, and it is connected to a comparator for generating the driving signal. The errors and defects of the turn-off timing of the driving signal may be solved and eliminated.