A 3-dimensional non-volatile memory device, a memory system including the same, and a method of manufacturing the same comprise vertical channel layers protruding from a substrate, a plurality of interlayer insulating layers and a plurality of conductive layers alternately formed along the vertical channel layers, a charge trap layer surrounding the vertical channel layers, the charge trap layer having a smaller thickness in a plurality of first regions, interposed between the plurality of conductive layers and the vertical channel layers, than in a plurality of second regions, interposed between the plurality of interlayer insulating layers and the vertical channel layers and a blocking insulating layer formed in each of the plurality of first regions, between the plurality of conductive layers and the charge trap layer.