A cannulation simulator. The simulator comprises a frame (1) shaped like a part of the human body, on the surface of which a cannulation zone (4) is arranged for insertion of a needle, in particular a cannulation needle. According to the invention, the cannulation zone (4) comprises a layer formed of a gel-like, at least essentially transparent polymeric material, which layer in regard to its mechanical properties at least approximately corresponds to human loose connective tissue, at least one vein simulating polymeric hose (5) arranged inside the polymeric material layer, as well as an elastic polymeric film (6) covering the polymeric material layer on the surface of the body. The simulator facilitates cannulation training.