A driving circuit is adapted to drive a current-driven device. The driving circuit includes a first power supply circuit and a second power supply circuit. The first power supply circuit is for supplying a first positive voltage to a first terminal of the current-driven device. The second power supply circuit is for enabling a current flowing along a first current flow direction in a first time period and thereby a second terminal of the current-driven device is given a second positive voltage. The second power supply circuit further is for enabling a current from the current-driven device flowing out of the second power supply circuit along a second current flow direction. The first current flow direction and the second current flow direction are different directions in the second power supply circuit. Moreover, a light emitting device using the above-mentioned driving circuit also is provided.