Invention Grant
US08663909B2 Device for rapid detection and identification of single microorganisms without preliminary growth
失效
用于快速检测和鉴定单一微生物而不进行初步生长的装置
- Patent Title: Device for rapid detection and identification of single microorganisms without preliminary growth
- Patent Title (中): 用于快速检测和鉴定单一微生物而不进行初步生长的装置
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Application No.: US11109857Application Date: 2005-04-20
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Publication No.: US08663909B2Publication Date: 2014-03-04
- Inventor: Sergey Gazenko
- Applicant: Sergey Gazenko
- Applicant Address: US OH Hubbard
- Assignee: Nanologix, Inc.
- Current Assignee: Nanologix, Inc.
- Current Assignee Address: US OH Hubbard
- Agency: Acker Wood IP Law, LLC
- Agent Gwen R. Acker Wood
- Main IPC: C12Q1/00
- IPC: C12Q1/00 ; C12Q1/02 ; C12Q1/04 ; C12M1/36 ; C12M1/38 ; C12M1/00 ; C12M3/00 ; C12M1/34 ; C12N11/00 ; C12N11/16 ; G01N33/53 ; G01N33/569 ; G01N33/554 ; G01N33/543

Abstract:
This invention describes a device consisting of a micro channel plate, filter, and porous holder for filter, which is substituted by a pure agar block during method performance, and supportive structural elements. The device is intended for rapid detection and/or identification of microorganisms. Microorganisms are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides and attached to a filter from one side. A micro channel plate houses a multiplicity of micro channels (possible diameter of each channel=1-30 μm, length 100-1000 μm, and number on centimeter2=100,000-1,000,000). The micro channel plate with cells trapped on the surface of the filter is attached to an agar block impregnated by artificial substrate(s) so that the molecules of the artificial substrates will fill all micro channels. Trapped cells produce colored or fluorescent molecules from artificial substrates. These molecules are collected in the very small volume of a micro channel. The extremely small volume of a micro channel (1/25 million part of milliliter) allows it to collect a detectable concentration of color or fluorescent substances in a very short time (several minutes). Even one cell from a filtrated sample can be detected by the enzyme—artificial substrate method and/or identified by enzyme immunoassay.
Public/Granted literature
- US20050221403A1 Device for rapid detection and identification of single microorganisms without preliminary growth Public/Granted day:2005-10-06
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