Invention Grant
US08524681B2 siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof
有权
用于减少RNAi中的靶外表型效应的siRNA序列非依赖性修饰形式及其稳定形式
- Patent Title: siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof
- Patent Title (中): 用于减少RNAi中的靶外表型效应的siRNA序列非依赖性修饰形式及其稳定形式
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Application No.: US12727336Application Date: 2010-03-19
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Publication No.: US08524681B2Publication Date: 2013-09-03
- Inventor: Nitin Puri , Irudaya Charles , Susan Magdaleno , Alexander Vlassov , Chris Burnett
- Applicant: Nitin Puri , Irudaya Charles , Susan Magdaleno , Alexander Vlassov , Chris Burnett
- Applicant Address: US CA Carlsbad
- Assignee: Applied Biosystems, LLC
- Current Assignee: Applied Biosystems, LLC
- Current Assignee Address: US CA Carlsbad
- Main IPC: C07H21/02
- IPC: C07H21/02 ; C07H21/04 ; A61K31/70 ; C12Q1/68
Abstract:
Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
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