An anti-reflection film which has not only sufficient anti-reflection properties and antistatic properties but also excellent optical properties is provided. The anti-reflection film includes an antistatic hard coat layer and a low refractive index layer on a transparent substrate, the antistatic hard coat layer containing conductive particles and a binder matrix, the antistatic hard coat layer including a mixed layer in which the transparent substrate component and the binder matrix blend together with a gradient and a localized layer, the mixed layer being optically indistinguishable and the localized layer being optically distinguishable, and the localized layer having an optical thickness of 50-400 nm.